Accurate in vivo tumor detection using plasmonic-enhanced shifted-excitation Raman difference spectroscopy (SERDS)
P. Strobbia1,2, V. Cupil-Garcia1,3, B.M. Crawford1,2, A.M. Fales4, T.J. Pfefer4, Y. Liu1,2, M. Maiwald5, B. Sumpf5, T. Vo-Dinh1,2,3
Published in:
Theranostics, vol. 11, no. 9, pp. 4090-4102, doi:10.7150/thno.53101 (2021).
Abstract:
For the majority of cancer patients, surgery is the primary method of treatment. In these cases, accurately removing the entire tumor without harming surrounding tissue is critical; however, due to the lack of intraoperative imaging techniques, surgeons rely on visual and physical inspection to identify tumors. Surface-enhanced Raman scattering (SERS) is emerging as a non-invasive optical alternative for intraoperative tumor identification, with high accuracy and stability. However, Raman detection requires dark rooms to work, which is not consistent with surgical settings.
Methods: Herein, we used SERS nanoprobes combined with shifted-excitation Raman difference spectroscopy (SERDS) detection, to accurately detect tumors in xenograft murine model.
Results: We demonstrate for the first time the use of SERDS for in vivo tumor detection in a murine model under ambient light conditions. We compare traditional Raman detection with SERDS, showing that our method can improve sensitivity and accuracy for this task.
Conclusion: Our results show that this method can be used to improve the accuracy and robustness of in vivo Raman/SERS biomedical application, aiding the process of clinical translation of these technologies.
1 Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA
2 Department of Biomedical Engineering, Duke University, Durham, NC, USA
3 Department of Chemistry, Duke University, Durham, NC, USA
4 Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD, USA
5 Ferdinand-Braun-Institut, Leibniz-Institut für Höchstfrequenztechnik, Berlin, Germany
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